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Purification of Ph αβ from the venom of the spider P. nigriventer (A) Reverse phase of the crude venom of Phoneutria nigriventer spider in column C4 Vydac. (B) Cation <t>exchange</t> <t>HPLC</t> of the fraction Phαβ from (A) on Hi trap Sp Hp column (C) Reverse phase desalting of Phαβ from (B) in a column C18 small pore (arrow indicates the peak used in each step) (D) Mass spectra of the Phαβ MS obtained on MALDI/TOF in positive/linear mode (laser frequency 100%, mass range 2–20,000 ( m/ z).
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Purification of Ph αβ from the venom of the spider P. nigriventer (A) Reverse phase of the crude venom of Phoneutria nigriventer spider in column C4 Vydac. (B) Cation <t>exchange</t> <t>HPLC</t> of the fraction Phαβ from (A) on Hi trap Sp Hp column (C) Reverse phase desalting of Phαβ from (B) in a column C18 small pore (arrow indicates the peak used in each step) (D) Mass spectra of the Phαβ MS obtained on MALDI/TOF in positive/linear mode (laser frequency 100%, mass range 2–20,000 ( m/ z).
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Purification of Ph αβ from the venom of the spider P. nigriventer (A) Reverse phase of the crude venom of Phoneutria nigriventer spider in column C4 Vydac. (B) Cation <t>exchange</t> <t>HPLC</t> of the fraction Phαβ from (A) on Hi trap Sp Hp column (C) Reverse phase desalting of Phαβ from (B) in a column C18 small pore (arrow indicates the peak used in each step) (D) Mass spectra of the Phαβ MS obtained on MALDI/TOF in positive/linear mode (laser frequency 100%, mass range 2–20,000 ( m/ z).
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Purification of Ph αβ from the venom of the spider P. nigriventer (A) Reverse phase of the crude venom of Phoneutria nigriventer spider in column C4 Vydac. (B) Cation <t>exchange</t> <t>HPLC</t> of the fraction Phαβ from (A) on Hi trap Sp Hp column (C) Reverse phase desalting of Phαβ from (B) in a column C18 small pore (arrow indicates the peak used in each step) (D) Mass spectra of the Phαβ MS obtained on MALDI/TOF in positive/linear mode (laser frequency 100%, mass range 2–20,000 ( m/ z).
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Purification of Ph αβ from the venom of the spider P. nigriventer (A) Reverse phase of the crude venom of Phoneutria nigriventer spider in column C4 Vydac. (B) Cation exchange HPLC of the fraction Phαβ from (A) on Hi trap Sp Hp column (C) Reverse phase desalting of Phαβ from (B) in a column C18 small pore (arrow indicates the peak used in each step) (D) Mass spectra of the Phαβ MS obtained on MALDI/TOF in positive/linear mode (laser frequency 100%, mass range 2–20,000 ( m/ z).

Journal: Frontiers in Molecular Biosciences

Article Title: Mapping of Brain Activity in the Analgesia Induced by Ph α 1 β and Morphine

doi: 10.3389/fmolb.2021.770471

Figure Lengend Snippet: Purification of Ph αβ from the venom of the spider P. nigriventer (A) Reverse phase of the crude venom of Phoneutria nigriventer spider in column C4 Vydac. (B) Cation exchange HPLC of the fraction Phαβ from (A) on Hi trap Sp Hp column (C) Reverse phase desalting of Phαβ from (B) in a column C18 small pore (arrow indicates the peak used in each step) (D) Mass spectra of the Phαβ MS obtained on MALDI/TOF in positive/linear mode (laser frequency 100%, mass range 2–20,000 ( m/ z).

Article Snippet: We collected three fractions (A–C; ), and fraction C was dissolved in 1 ml of 10 mM sodium phosphate buffer, pH 6.5, and fractionated on a weak cation exchange HPLC column (4.6 mm _ 25 cm) of Synchropak CM 300 (Synchrom Inc, Lafayette, IN) equilibrated in the same buffer.

Techniques: Purification